PCR vs DNA Sequencing

1. DNA sequencing  require DNA polymerase, primers, dNTPs (deoxynucleoside triphosphates).
PCR require Taq polymerase, primers, supply of free nucleotides.

2. DNA sequencing require radioactively labelled dideoxy analogue of one of the DNTPs to be placed in each tube. Dideoxy analogue stops replication.

In PCR, the replication stops by itself because the polymerase reaches an end.

3. DNA Sequencing is mostly used for human genome project or sequencing a specie. 

PCR is mostly used for amplifying genes for example in forensic science. 

4. PCR can be operated in one container. Everything duplicates in one test tube for example.

DNA sequencing requires 4 separate test tube because each dideoxy analogue must be put in separate compartment. 

5. PCR starts with double stranded DNA sequence then it is heated to separate and start replication.

DNA sequence starts with a primed single-stranded DNA.